B.Pharmacy 7th Semester Instrumental Methods of Analysis Notes & Important Question Answer
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| B.Pharmacy 7th Semester Instrumental Methods of Analysis Notes |
Instrumental Methods of Analysis - Unit 1
UV Visible spectroscopy
- Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
- Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors - Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
- Applications - Spectrophotometric titrations, Single component and multi component analysis
Fluorimetry
- Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications
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Instrumental Methods of Analysis - Unit 2
IR spectroscopy
- Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations
- Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications
- Flame Photometry - Principle, interferences, instrumentation and applications
- Atomic absorption spectroscopy - Principle, interferences, instrumentation and applications
- Nepheloturbidometry - Principle, instrumentation and applications
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Instrumental Methods of Analysis - Unit 3
Introduction to chromatography
- Adsorption and partition column chromatography - Methodology, advantages, disadvantages and applications.
- Thin layer chromatography - Introduction, Principle, Methodology, Rf values, advantages, disadvantages and applications.
- Paper chromatography - Introduction, methodology, development techniques, advantages, disadvantages and applications
- Electrophoresis – Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel, capillary electrophoresis, applications
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Instrumental Methods of Analysis - Unit 4
- Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications
- High performance liquid chromatography (HPLC) - Introduction, theory, instrumentation, advantages and applications.
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Instrumental Methods of Analysis - Unit 5
- Ion exchange chromatography - Introduction, classification, ion exchange resins, properties, mechanism of ion exchange process, factors affecting ion exchange, methodology and applications
- Gel chromatography - Introduction, theory, instrumentation and applications
- Affinity chromatography - Introduction, theory, instrumentation and applications
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Instrumental Methods of Analysis book PDF Downloading Link
- Instrumental Methods of Analysis Nirali Publication Full Book PDF:- Click Here
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B Pharmacy 7th Semester Notes
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